Fig. 1

Expression of SUMO1 and SENP1 in DRG neurons. a Distribution of SUMO1 in DRG neurons. DRG neurons were permeabilized and double labeled with SUMO1 (red) and NF200, peripherin, IB4 and CGRP (green). The percents of SUMO1-positive neurons among those labeled with the indicated markers are shown in the bar graph below. The total numbers of neurons analyzed (n) ranged from 48 to 89 cells per condition. Data are means ± s.e.m, from three experiments. Scale bar: 100 μm. b Distribution of SENP1 in DRG neurons. Similar to a but SENP1 was used in place of SUMO1. The total numbers of neurons analyzed (n) ranged from 64 to 117 cells per condition. Data are means ± s.e.m, from three experiments. Scale bar: 100 μm. c RNA analysis by RT-PCR of TRPV1, SUMO1, SENP1, and GAPDH levels in DRG and spinal cord from adult wild-type mice. Negative controls: samples without reverse transcriptase (−RT) and water (W). Gel images are representatives of three independent experiments. d SUMO1-conjugated proteins were increased in DRG in the ipsilateral side of carrageenan-injected hindpaw as compared to the contralateral side. Carrageenan (2%, 20 μl) was administered by intraplantar injection into the left hindpaw of wild-type mice. L3–L4 DRG at the left (Ipsi) and right (Contra) sides were dissected 1 h after the injection and SUMO1-conjugated proteins were measured by western blotting. GAPDH was used as the loading control. Blots are representatives of three independent experiments. e SUMO1-conjugated proteins were increased in DRG but not spinal cord. At 0, 1, or 2 h after intraplantar injection of carrageenan (Carra.) into both hindpaws, L3–L4 DRG from both sides and spinal cord were dissected for western blotting for SUMO1 and GAPDH. Blots are representatives of three independent experiments