Fig. 2

Generation of primary somatosensory neuron-specific SENP1 cKO mice. a PCR analysis of genomic DNA obtained from DRG of SENP1^flox/flox and Prrxl1-CreER^T2 intercrosses. Line 1, wild type; line 2, SENP1^flox/flox (Control); line 3, Prrxl1-Cre ER^T2; line 4, SENP1^flox/flox; Prrxl1-Cre ER^T2 (SENP1 cKO); line 5, SENP1^wt/flox; Prrxl1-Cre ER^T2. b SENP1 mRNA was reduced in DRG of SENP1 cKO mice. Real-time qPCR was used to determine the SENP1 mRNA levels in the DRG and spinal cord from SENP1 cKO and its littermate control mice 4 weeks after tamoxifen administration. Data are mean ± s.e.m. normalized to that of the control from three independent experiments. P = 0.0227, Control DRG vs. cKO DRG by Student’s t test. c SUMO1-conjugated proteins were increased in DRG, but not spinal cord, of SENP1 cKO mice. DRG and spinal cord were dissected from SENP1 cKO and their littermate control mice and analyzed by western blotting using anti-SUMO1 and anti-GAPDH antibodies. d SENP1 protein level was decreased in SENP1 cKO DRG compared to its littermate controls. DRG and spinal cord were dissected from SENP1 cKO and their littermate control mice and analyzed by western blotting using anti-SENP1 and anti-GAPDH antibodies. e Verification of SENP1 gene deletion in DRG of SENP1 cKO mice by immunohistochemistry, with double staining for SENP1 (red) and peripherin (green) or NF200 (green) for DRG sections from SENP1 cKO mice (lower) and its littermate controls (upper). Scale bar: 100 μm