Fig. 3

Increased inflammatory thermal hyperalgesia in SENP1 cKO mice. a Locomotor activities and exploratory behaviors of uninjured 8-week-old SENP1 cKO mice and its littermate controls were assessed by open field test. Total distances traveled during 30 min were: 96.6 ± 12.4 m for control (n = 14) and 101.6 ± 9.2 m for SENP1 cKO (n = 6). b Motor coordination skills for SENP1 cKO and their littermate control mice were assessed by rotarod test. The average latencies to fall from the rotating bar for three consecutive trials were: 261.7 ± 8.3 s for control (n = 7) and 249.7 ± 10.5 s for SENP1 cKO mice (n = 10). c Nocifensive behaviors in response to intraplantar injection of capsaicin (2 μg) were tested for SENP1 cKO and their littermate control mice. The time spent on licking, biting, and lifting (nocifensive behaviors) within the first 10 min of capsaicin injection were: 85.0 ± 6.0 s for control (n = 8) and 74.3 ± 6.4 s for SENP1 cKO mice (n = 12). d Baseline withdrawal latencies of hindpaws to radiant heat were measured for SENP1 cKO, its littermate controls and TRPV1^−/− mice by Hargreaves’s method. The paw withdrawal latencies (PWL) were: 10.51 ± 0.31 s for control (n = 15), 9.43 ± 0.55 s for SENP1 cKO mice (n = 8), and 10.79 ± 0.31 s for TRPV1^−/− (n = 5). e Time courses of PWL as assessed by the Hargreaves’s method before (0 min) and at different time after intraplantar injection of carrageenan (2%, 20 μl) into the left hindpaws of TRPV1^−/− mice, SENP1 cKO mice and their littermate controls. TRPV1^−/− mice did not develop inflammatory thermal hyperalgesia, whereas SENP1 cKO mice exhibited markedly decreased PWL as compared to the control in the carrageenan edema model (Control, n = 15; SENP1 cKO, n = 8; TRPV1^−/−, n = 5). ***P < 0.0001, Control vs. SENP1 cKO; ***P < 0.0001, Control vs. TRPV1^−/−. f Time courses of PWL as assessed by the Hargreaves’s method before (0 min) and at different times after intraplantar injection of CFA (0.5 mg ml⁻¹, 20 μl) into the left hindpaws of SENP1 cKO mice and their littermate controls. Both the left (Ipsi) and right (Contra) paws were tested (Control, n = 12; SENP1 cKO, n = 9). ***P < 0.0001, Control Ipsi vs. SENP1 cKO Ipsi; ***P < 0.0001, Control Contra vs. Control Ipsi, and ***P < 0.0001, SENP1 cKO Contra vs. SENP1 cKO Ipsi. Data are means ± s.e.m. Two-way ANOVA for e and f