Fig. 5
From: TRPV1 SUMOylation regulates nociceptive signaling in models of inflammatory pain
K822R mutation of TRPV1 did not alter its membrane surface expression and capsaicin-evoked whole-cell current density. a CHO-K1 cells were transfected with Flag-TRPV1 or Flag-TRPV1-K822R. Representative traces (left) and summary of current density (right) for capsaicin (1 μM)-activated whole-cell currents at −60 mV are shown. The current densities are: TRPV1, 345 ± 56 pA/pF (n = 7) and TRPV1-K822R, 398 ± 59 pA/pF (n = 5). Data are means ± s.e.m. b CHO-K1 cells were transfected with Flag-TRPV1 or Flag-TRPV1-K822R. Surface levels of TRPV1 were measured by IB after plasma membrane proteins were biotinylated and purified with streptavidin-agarose. GAPDH were used as controls for cytoplasmic proteins, respectively. Blots are representatives of at least three independent experiments
