Fig. 4
From: Quantitative diffusion measurements using the open-source software PyFRAP
Examples of in vitro FRAP and iFRAP experiments and the resulting fits to measure free diffusion. a, c, e, g, i In vitro FRAP and iFRAP experiment images and b, d, f, h, j fits with PyFRAP. Black and grey dots represent data points of bleached and slice ROI, respectively. Red solid and dashed lines show the respective fits. a–d FRAP experiments with 3 and 70 kDa fluorescent dextrans (see Supplementary Fig. 5 for the full data set with fluorescent dextrans between 3 and 500 kDa). e–h iFRAP experiment with photoconverted Dendra2 protein showing data for the green (e, f) and the red (g, h) channel. i, j FRAP experiment with 70 kDa fluorescent dextran in the presence of polyacrylamide beads. Recovery curves were normalised between 0 (intensity in the bleached ROI at the first post-bleach time point) and 1 (intensity in the bleached ROI at the last post-bleach time point) to allow comparison across data sets. The length of the white scale bar in a represents 100 μm, and all images were acquired with the same magnification
