Fig. 3
From: Gene refashioning through innovative shifting of reading frames in mosses
Quantification of fatty acids and wax loads in wild-type plants and yan/altyan mutants. Ten-week old plants were used to detect the composition and total amount of fatty acids and waxes. Results represent means and standard deviations of three biological replicates for the wild-type and two independent mutants. Asterisks indicate a statistically significant difference compared with wild-type plants based on a two-tailed Student’s t test (p < 0.05). a Total fatty acid content in the wild-type and yan/altyan. b Fatty acids composition in wild-type plants and yan/altyan mutants. c Wax composition in wild-type plants and yan/altyan mutants. 1. 16-Hydroxy ent-kaurane; 2. 1-Tetracosanol; 3. 1-Hexacosanol; 4. Octadecane-1-bromo; 5. Kaur-15-ene; 6. Ent-kaurene; 7. 1,19-Eicosadiene; 8. 1,21-Docosadiene; 9. 1,21-Tetracosene; 10. Octadecanal; 11. Eicosenel; 12. Docosanal; 13. Tetracosanal. d Wax crystals observed using scanning electron microscopy. Scale bar = 2 μm
