Fig. 5
From: Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s
Configuration of the KLP10A neck domain. a, b Structure of the neck domain (ND) in the NMMT complexes. a NMMTapo. b NMMTAMP-PNP. c Class averages of curved tubulin protofilaments obtained by aligning and classifying whole ring-like structures observed in cryo-EM images (Supplementary Fig. 1d). d, e Class averages of curved tubulin protofilaments obtained by aligning/classifying regions containing two tubulin heterodimers from the classes shown in c. This classification produced class-averages with one KLP10A motor domain per tubulin heterodimer (d) and class averages with one KLP10A motor domain per two tubulin heterodimers (e). f In the class average images with only one KLP10A motor domain per two tubulin heterodimers, an elongated density can be observed going from the motor domain density (M) to the density corresponding to the next tubulin heterodimer (α-2, β2). g The elongated density in f is modeled as an α-helix formed by the KLP10A neck-domain (ND)
