Fig. 1
From: RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
Targeted gene silencing of endogenous Pcsk9 in vitro. a Deactivated S. aureus dCas9 was fused to a KRAB repressor motif and delivered by lentivirus for in vitro gRNA screening. The lentiviral vector also contained a puromycin resistance gene and a gRNA expression cassette. b A panel of eight S. aureus gRNAs were designed to target the accessible chromatin region of the mouse Pcsk9 promoter region in AML12 cells, a mouse hepatocyte cell line with high expression of Pcsk9. An ENCODE wild-type mouse liver DNase I hypersensitivity-sequencing track is included to highlight the accessible chromatin region around the Pcsk9 transcription start site38. c Single gRNAs were screened for silencing efficacy by qRT-PCR. (mean ± s.e.m., n = 2 biological replicates). P < 0.05 indicated by * compared with the non-transduced (NT) control (Student’s t-test)
