Fig. 3
From: RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
Genome-wide analysis of gene expression in dSaCas9KRAB-treated mice. a–d RNA-sequencing and differential expression analysis was performed comparing liver tissue from mice treated with a, b AAVs expressing dSaCas9KRAB and Pcsk9-targeting gRNA vs. dSaCas9KRAB alone, c AAVs expressing dSaCas9KRAB with Pcsk9-targeting gRNA to PBS, or d AAV expressing dSaCas9KRAB vs. PBS. Red data points indicate FDR < 0.05 by differential-expression analysis with a negative binomial generalized linear model and Wald test for significance52 (n = 4, mice 6–8 weeks old at time of treatment). The data point representing the Pcsk9 transcript is highlighted in blue (FDR > 0.05). Extended gene lists found in Supplementary Data 1, 4, 5
