Fig. 4
From: RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
Transient effects of AAV-mediated dSaCas9KRAB gene silencing on host liver. a Secreted alanine transaminase as a measure of liver toxicity was assayed in the serum of treated mice over 6 weeks post-treatment (mean ± s.e.m., n = 4 mice at 6–8 weeks old when treated). P < 0.05 is indicated by * and calculated by mixed design ANOVA with Tukey’s post-hoc analysis compared to day 0 controls. Dotted line marks the upper limit of physiological levels of ALT in adult C57Bl/6 mice. b H&E staining was performed on sections from livers of mice harvested 6 weeks post-treatment (representative images shown, scale bars = 100 μm)
