Fig. 5
From: RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
Long-term efficacy of targeted transcriptional silencing with dSaCas9KRAB. In mice treated with AAVs expressing dSaCas9KRAB and Pcsk9-targeting gRNA at 2×1011 and 4×1011 viral genomes/vector/mouse, serum was collected over 168 days post-treatment and analyzed for a alanine transanimase secretion, b Pcsk9 protein concentration, and c low-density lipoprotein-cholesterol concentration (mean ± s.e.m., n = 4 mice at 6–8 weeks old when treated). * and # mark P < 0.05 in 2e11 and 4e11 doses respectively, calculated by mixed design ANOVA with Tukey’s post-hoc analysis compared to PBS controls
