Fig. 1
The resolution limit caused by the effect of depth of field. a Positions marked with a cross (x) represent the calculated resolution limit of protein complexes of variable sizes by using the simulated 300 kV (black), 200 kV (red), and 120 kV (blue) cryo-EM data. The corresponding black, red, and blue lines are the resolution limits calculated using the modified empirical formula. Values for the dashed black line were obtained by applying the DeRoiser’s empirical formula that was used as a reference for comparison21, 22. The black-edged square is the simulation result performed by Kenneth et al.23. b Black and red circles with EMDB codes represent the resolutions of typical high-resolution protein complexes generated by cryo-EM SPA at 300 kV and 200 kV, respectively. Black squares represent the resolutions of PBCV-1 virus and HSV-2 virus that were reconstructed by using a block-based reconstruction method. Gray squares represent the resolution of these two viruses that were reconstructed by using a conventional method
