Fig. 3
From: Repeated evolution of self-compatibility for reproductive assurance
Fitness benefits and costs of mating-type switching (MTS) during sexual and asexual reproduction. a Selection coefficients for non-switchers (NS), relative to switchers (SW), after one round of sexual reproduction under four different densities in a structured environment (8.0 × 105 cells mm−2 in highest density) on inverted y-axis to indicate reduction in fitness. Non-switchers decrease in fitness at low densities and the benefit of switching is the highest when switchers are at high frequency in the population, regardless of density. The boxplots are based on eight biological replicates (open circles; exceptions are indicated by number above box; see Methods). All strains competing are isogenic, except for mCherry marker and switching locus. Asterisks indicates significant deviation from the control (Dunn’s Kruskal-Wallis multiple comparison, *p < 0.05, **p < 0.01, ***p < 0.001). b Per generation selection coefficient for unlabelled switcher, relative to mCherry labelled non-switchers of M, P or a mixture of equal numbers of P and M, under asexual growth after 5 days of exponential growth (~45 generations; left panel) or after five transfers of growth to saturation (~33 generations; right panel). Control is the competition between the wild-type strain h90 (EBC5) and itself but mCherry labelled (EBC47). Each boxplot consists of eight technical replicates (grey dots). Letters within each panel indicate significantly different groups based on Dunn’s Kruskal–Wallis multiple comparison (p < 0.05). Note inverted y-axis
