Fig. 1 | Nature Communications

Fig. 1

From: Native mass spectrometry combined with enzymatic dissection unravels glycoform heterogeneity of biopharmaceuticals

Fig. 1

Molecular structure and native mass spectrometry of Etanercept. a Schematic illustration of dimeric Etanercept consisting of a TNFR and an Fc domain. Disulfide bonds in the Fc region are indicated as yellow lines; disulfide bridges in the TNFR domain are not shown. Monosaccharide symbols are listed. Exemplary cleavage sites of IdeS, PNGase F and sialidase are indicated. Native mass spectra of b intact Etanercept (Rset = 17,500 at m/z 200), d Etanercept digested with sialidase or f PNGase F (Rset = 35,000 at m/z 200), and h a combination of PNGase F/sialidase, respectively (Rset = 70,000 at m/z 200). Charge states are indicated. Zooms into the most abundant charge states are shown in c, e, g, and i

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