Fig. 3 | Nature Communications

Fig. 3

From: Native mass spectrometry combined with enzymatic dissection unravels glycoform heterogeneity of biopharmaceuticals

Fig. 3

N-glycosylation of Etanercept TNFR and Fc domains. a Deconvoluted spectrum of dimeric TNFR digested with sialidase and O-glycosidase acquired under native conditions (raw spectrum shown in Supplementary Fig. 6c, d). The most probable glycan structures lacking sialic acids are annotated. b Deconvoluted spectrum of Fc dimer upon native MS (raw spectrum shown in Supplementary Fig. 7). The most probable N-glycoforms and C-terminal lysine variants are annotated. Asterisks indicate Na+ adducts. Peak lists with all possible glycoform assignments are available in Supplementary Data 1

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