Fig. 4
N- and O-glycosylation of Etanercept lacking sialic acids. a Deconvoluted spectrum of Etanercept treated with sialidase and O-glycosidase acquired under native conditions (raw spectrum is shown in Supplementary Fig. 8b). The most probable glycan structures lacking sialic acids are annotated. The six most abundant N-glycoforms are boxed and marked as A to F, respectively. b Deconvoluted spectrum of sialidase-treated Etanercept upon native MS (raw spectrum is shown in Fig. 1d). The most probable glycoforms are annotated. N-glycan structures are referred to as A to F as specified in Fig. 4a; O-glycoforms are labeled according to Fig. 2a. Peak lists with all possible glycoform assignments are available in Supplementary Data 1
