Fig. 1
From: Barium bioaccumulation by bacterial biofilms and implications for Ba cycling and use of Ba proxies
SEM images of cells and EPS from I. loihiensis and the bioaccumulated Ba. a Cells, EPS, and Ba precipitates after 48 h of incubation in SSW2 medium. The image was obtained by secondary electrons with the Inlens detector at 5 kV. b Same image shown in a in backscattered electron (BSE) mode by AsB detector at 20 kV. Scale bar in a and b represents 300 nm. c Representative SEM-EDX spectrum (analyzed spot marked with an arrow in BSE image), in which the vertical scale is enlarged to show the lower intensity of S peak in relation to P. Elements, other than those bound to EPS, derive from the glass coverslips used in the experiments (e.g. Si, K), see Supplementary Fig 1 for a background spectrum. d, e I. loihiensis MAH1 after 96 h of incubation, and the same conditions previously mentioned for photographs a and b. f Spectrum showing the enhanced intensity of the S peak in relation to P. Scale bar represents 200 nm. Photographs d and e and corresponding spectrum were obtained with a Zeiss SUPRA40VP and the rest with an AURIGA FIB-FESEM Carl Zeiss SMT
