Fig. 1

Adult and cord blood Vδ2⁺ TCR repertoires are broadly similar. a Tree maps show each adult donor’s Vδ2⁺ TCR repertoire, with each CDR3 clonotype as a coloured segment (each coloured CDR3 segment is chosen randomly and does not match between plots) plotted in relation to the total repertoire size and accompanying clonotype frequency graphs showing the individual clone frequency (left y axis) and the accumulated frequency for the 10 most prevalent clonotypes (right y axis). Inset into each graph are D75 repertoire diversity metrics (measuring the percentage of clonotypes required to occupy 75% of the total TCR repertoire). b Tree maps showing TCR γ and δ CDR3 clonotypes, accumulated frequency graphs and D75 metric from cord blood Vδ2⁺ T cells. c Jγ and d Jδ segment usage in Vδ2⁺ TCR repertoires from adult peripheral blood (n = 7) and cord blood samples (n = 4). e Logo analysis of amino acid enrichment at each position in neonatal Vδ2–Jδ1 CDR3δ (left) and Vδ2–Jδ3 CDR3δ (right) sequences. Analysis was confined to the 10 most abundant CDR3δ2 sequences of 13–16 amino acid length. The different amino acids are coloured according to physicochemical properties (acidic (red); basic (blue); hydrophobic (black); and neutral (green)). Red arrows indicate position 5 in the CDR3 sequence (see Methods section). f Comparison of accumulated frequency curves generated from the 10 most prevalent TCRγ (left) and δ (right) clonotypes in Vδ2⁺ and Vδ1⁺ TCR repertoires (Vδ1 cohort data analysed from¹¹) from adult peripheral blood (Vδ2⁺, n = 7 and Vδ1⁺, n = 13) and cord blood (Vδ2⁺, n = 4 and Vδ1⁺, n = 5). g Comparison of TCRγ D75 metrics from adult peripheral blood and cord blood Vδ2⁺ (adult: n = 7; cord blood: n = 4) and Vδ1⁺ repertoires (adult focused: n = 13; adult diverse: n = 7; cord blood: n = 5). h Comparison of the CDR3 length profiles in Vδ2⁺ TCRδ and γ repertoires from adult peripheral blood (n = 7) and cord blood (n = 4). Error bars indicate means ± SEM; **P < 0.01; ***P < 0.001; p-values were determined by Student's t-test (g: left) and Kruskal–Wallis test (ANOVA) with Tukey’s post hoc testing (g: right). NS not significant