Fig. 6
From: The human Vδ2+ T-cell compartment comprises distinct innate-like Vγ9+ and adaptive Vγ9- subsets
Vγ9− Vδ2+ T cells exhibit TCR-specific clonal expansion in response to viral infection. a Identification of Vδ2+ T cells in CD3+ T cells (top row) and Vγ9− cells within Vδ2+ T cells (bottom row) in longitudinal peripheral blood samples from two CMV-seronegative kidney transplant patients developing post-operative acute CMV infection (CMV-seroconversion: patient 231, 8 weeks; patient 261, 7 weeks). b CD27 and CD45RA T-cell memory marker expression profiles by detectable Vγ9− Vδ2+ T cells populations in longitudinal peripheral blood samples from patients 231 and 261. Cytometry data from patient 261 at 281 weeks was acquired on an alternative flow cytometer (a and b). c Single-cell CDR3δ sequence analysis and Vγ usage by Vγ9− Vδ2+ T cells sorted from patients 231 and 261 peripheral blood samples between 39 and 328 weeks after transplantation. d Analysis of the indicated naive and effector T-cell markers by Vγ9− Vδ2+ T cells from patients 261 (week 48) and 231 (week 328) and in comparison with CD27hi, CD27lo/neg Vδ1+ and Vγ9− Vδ2+ T cells from the peripheral of blood of two healthy donors analysed in parallel. ND no detectable population
