Fig. 3

Comparison of three enrichment methods. a Optimization of the concentrations of TFA as the ion-pairing reagent for ZIC-HILIC enrichment. b The numbers of unique glycopeptides (dark blue) and glycoproteins (green) identified using each of the three methods (lectin, ZIC-HILIC, and DBA) from parallel experiments. c Comparison of the enrichment specificity for three enrichment methods. The error bar represents the standard error of the mean calculated from triplicate experiments