Fig. 5
From: Exercise induces new cardiomyocyte generation in the adult mammalian heart
Hypoxia and hippo pathways are not directly targeted by miR-222. a, b qPCR was used to analyze mRNA levels of genes relevant for hypoxia-induced effects in exercised vs. sedentary hearts (a) and hearts from mice treated with LNA-anti-miR222 or LNA-scr-miR undergoing voluntary wheel running (b) (n = 3–5 mice per group, *p < 0.05, Student’s t test). c p value from significantly differential pathways analyzed by ingenuity pathway analysis (IPA, Qiagen) of a microarray conducted from neonatal rat ventricular myocytes (NRVM) treated with control precursor (ctl-pre) and miR-222 precursor (pre-miR-222), respectively (n = 4 individual samples per group). d, e qPCR was used to analyze mRNA levels of genes relevant for hippo pathway-induced effects in exercised vs. sedentary hearts (d) and hearts from mice treated with LNA-anti-miR222 or LNA-scr-miR undergoing voluntary wheel running (e) (n = 3–5 mice per group, *p < 0.05, Student’s t test). f Gene expression signal specifically for CCGN2 and TEAD2 detected in a microarray conducted from neonatal rat ventricular myocytes (NRVM) treated with control precursor (ctl-pre) and miR-222 precursor (pre-miR-222), respectively (n = 4 individual samples per group, *p < 0.05, Student’s t test). Error bars represent ± s.e.m
