Fig. 2 | Nature Communications

Fig. 2

From: Aurora A-dependent CENP-A phosphorylation at inner centromeres protects bioriented chromosomes against cohesion fatigue

Fig. 2

CENP-A-S7P is required for the protection of sister chromatid cohesion during mitosis. a After endogenous CENP-A siRNA depletion, metaphase chromosome spreads from CENP-A-HA-WT, CENP-A-HA-S7A, or CENP-A-HA-S7D cell lines were immunostained with an anti-HA antibody. A × 9 magnification of a single chromosome is shown. Scale bar = 5 µm. b Mitotic cells displaying premature sister chromatid separation (PSCS) were quantified with two different clones from each cell line; at least 1000 cells were counted for each data point. The data shown are the mean ± SD (n = 3 experiments; **P<0.01, ***P<0.001, one-way ANOVA)

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