Fig. 2
From: Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage
Box H/ACA but not C/D snoRNA require cap structure. Transcriptional induction of a box C/D SNR13, b box H/ACA SNR3 c and protein-coding GAL1 in ceg1-63 versus WT strains; Northern blot analysis. Cells were shifted to the non-permissive temperature for 15 min prior to galactose induction and were incubated at this temperature during transcriptional pulse. Pa precursors with long polyadenylated 3ʹextensions, Me precursors with short oligoadenylated 3ʹextensions, M mature, Mt 5ʹtruncated mature snoRNA. d Quantification of synthesis rates for snR13, snR3 and GAL1 mRNA in WT and ceg1-63 strains normalised to the loading controls. Values for WT were set to 100%. e Transcriptional induction profiles of RD box C/D SNR65 and box H/ACA SNR43; mature and precursors snoRNA are marked as above. Northern blot analysis. f RNA-seq analysis of total RNA from cbp20Δ cbp80Δ strain. The X axis values are independently adjusted for each sample. Green and light green rectangles denote mature snoRNA and adjacent protein-coding genes, respectively
