Fig. 3 | Nature Communications

Fig. 3

From: Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage

Fig. 3

Lack of the 5ʹprocessing affects box C/D snoRNA 3ʹend maturation. RNA-seq analysis of a RD box C/D snoRNA and b Rnt1-independent box C/D snR13 as well as RD box H/ACA snoRNA 3ʹends in WT versus rnt1Δ strains. Metagene analyses are shown on the right. Dotted vertical lines denote mature 3ʹend. c, d CR-RT-PCR analysis as shown in diagram, for snR68 and snR65 in rnt1Δ and WT strain. Red vertical lines separate mature 3ʹends from 3ʹextension (post-transcriptionally added oligo(A) in red). e, f RNA-seq analysis of specified snoRNA 3ʹends in tgs1Δ and rnt1Δ/tgs1Δ strains. SnoRNA used for metagene analysis are listed in Supplementary Table 3

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