Fig. 4
From: Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage
Capped 5ʹextension affects box C/D pre-snoRNA 3ʹend processing. a Northern blot of WT snR68 or mutated in the stem-loop. Oligonucleotides used for RNase H cleavage and detection are indicated on the left. b Northern blot of SNR13 modified strains. Diagram on the left shows variants of endogenous SNR13 expressed from ectopic GAL1 promoter and oligonucleotides used for RNase H cleavage and detection. Asterisk marks incompletely digested snR13. c Northern blot analysis of SNR3 expressed from GAL1 promoter. d Northern blot analysis of snR52 transcribed from endogenous (Pol III-dependent) and GAL1 promoter in rnt1Δ and WT strains. e Northern blot and quantitation of snR13e transcripts in ceg1-63 strain. Amount of RNA loaded on the gel is indicated above the radiogram. Blue stripes on the hybridization profiles indicate the area covering 3ʹprocessed snR13e. f Northern blot of snR13e expressed in cbp80Δ strain. g RNA-seq showing snoRNA 3ʹends in rnt1Δ cbp80Δ strain. a–f RNA was treated with RNAse H to create homogenous 5ʹends. M denotes snoRNA with mature 3ʹends; Me denotes snoRNA with 3ʹend extensions
