Fig. 5
From: Nuclear fate of yeast snoRNA is determined by co-transcriptional Rnt1 cleavage
5ʹextended capped box C/D pre-snoRNA are mislocalized. Localization of a Rnt1-dependent box C/D snR68, b box H/ACA snR43 and c Rnt1-independent box C/D snR13 in rnt1Δ versus WT strains; FISH analysis. Probes against snoRNA mature sequence visualize both mature and pre-snoRNA, while probes complementary to the 5ʹextension detect only 5ʹextended precursors. d Nop1-GFP nuclear localization in the rnt1Δ strain. e Cellular localization of snR13 expressed from variant extensions of GAL1 promoter. The same probe against snR13 mature sequence was used for all strains. Nuclei were visualised by DAPI staining. DIC denotes differential interference contrast
