Fig. 4

CrmD binding affinity for mouse chemokines. a Binding screening of mouse chemokines to CrmD. Recombinant CrmD was immobilized
on a CM4 SPR biosensor chip at 1500 RU. Chemokines (100 nM) were injected at 10 µl/min flow rate in HBS-EP buffer. Binding was monitored during 180 s followed by a 120 s dissociation periods. b Binding kinetic constants K_a (association), K_d (dissociation) and K_D (affinity) and their corresponding SEM for the CrmD interaction with some of its chemokine ligands. For affinity determination purposes, CrmD was immobilized on a CM4 SPR biosensor chip at 900 RUs. The binding of increasing concentrations of chemokine (1–50 nM), injected at a 30 µl/min flow rate, was recorded during 120 s followed by a 300 s dissociation. Binding curves were fitted according to a 1:1 Langmuir model. c Two examples of affinity determination fittings are shown