Table 1 Antibodies used in this study
From: Bcl11b is essential for licensing Th2 differentiation during helminth infection and allergic asthma
Target | Clone | Source | Identifier | Concentration |
|---|---|---|---|---|
Anti-mouse CD4 | GK1.5 | Affymetrix | 48-0041 | 0.25 µg/100 μl |
Anti-mouse CD4 | GK1.5 | BioLegend | 100453 | 0.25 µg/100 μl |
Anti-mouse CD8a | 53-6.7 | Affymetrix | 25-0081 | 0.5 µg/100 μl |
Anti-mouse CD8a | 53-6.7 | Affymetrix | 47-0081 | 0.5 µg/100 μl |
Anti-mouse CCR9 | eBioCW-1.2 | Affymetrix | 11-1991 | 0.25 µg/100 μl |
Anti-mouse CD44 | IM7 | Affymetrix | 48-0441 | 0.25 µg/100 μl |
Anti-mouse CD62L | MEL-14 | Affymetrix | 11-0621 | 0.5 µg/100 μl |
Anti-mouse α4β7 | DATK32 | Affymetrix | 17-5887 | 0.25 µg/100 μl |
Anti-mouse CD11b | M1/70 | Affymetrix | 17-0112 | 0.125 µg/100 μl |
Anti-mouse CD11c | N418 | Affymetrix | 12-0114 | 0.5 µg/100 μl |
Anti-mouse GR-1 | 1A8-Ly6g | Affymetrix | 11-9668 | 0.5 µg/100 μl |
Anti-mouse Ly6C | HK1.4 | Affymetrix | 48-5932 | 0.25 µg/100 μl |
Anti-mouse SiglecF | E50-2440 | BD Pharmingen | 562680 | 0.2 µg/100 μl |
Anti-mouse IL-4 | 11B11 | BioLegend | 504125 | 0.4 µg/100 μl |
Anti-mouse IL-5 | TRFK5 | Affymetrix | 12-7052 | 0.25 µg/100 μl |
Anti-mouse IL-13 | eBio13A | Affymetrix | 53-7133 | 0.25 µg/100 μl |
Anti-mouse Gata3 | L50-823 | BD Pharmingen | 560068 | 20 µl/100 μl |
Anti-mouse Gata3 | 16E10A23 | BioLegend | 653808 | 5 µl/100 μl |
Anti-mouse Rorγ(t) | AFKJS-9 | Affymetrix | 12-6988 | 0.5 µg/100 μl |
Anti-mouse T-bet | 4B10 | BioLegend | 644816 | 0.4 µg/100 μl |
Anti-mouse Ki67 | SolA15 | Affymetrix | 11-5698 | 0.25 µg/100 μl |
Anti-mouse Runx3 | R3-5G4 | BD Pharmingen | 564814 | 5 µl/100 μl |
Fixable viability dye | N/A | Affymetrix | 65-0865 | 0.1 µl/100 μl |
Anti-mouse Runx3 | Polyclonal | See ref.63 | N/A | 5 μl/1000 μl (ChIP) |
Anti-mouse Ctip2 | 25B6 | abcam | ab18465 | 8 μg/1000 μl (ChIP) |
Anti-mouse Bcl11b | D6F1 | Cell Signaling Technologies | 12120 | 3 μg/1000 μl (ChIP) |
