Fig. 4
Depletion of C/EBPβ diminishes OGD or Aβ-induced delta-secretase expression. a, b Knockdown of C/EBPβ reduces delta-secretase expression induced by OGD. Immunoblotting and real-time PCR were conducted from primary cultures infected with virus expressing control or shRNA against C/EBPβ. c, d Knockdown of C/EBPβ reduces delta-secretase expression induced by Aβ. Western blot data in a, b are representative of three independent experiments. Data in c, d represent mean ± s.e.m. of three independent experiments (*P < 0.05, **P < 0.01, one-way ANOVA). e, f OGD and Aβ stimulate inflammatory cytokine expression, and knockdown of C/EBPβ reduces cytokine levels. Data represent mean ± s.e.m. of three independent experiments (*P < 0.05, one-way ANOVA). g, h Knockout of C/EBPβ significantly reduces delta-secretase expression in the brain. Wild-type or C/EBPβ KO mice (4–5 months old) were sacrificed and their brains were isolated. Delta-secretase’s mRNA (g) and protein (h) levels in the brains were analyzed by western blotting and real-time PCR. C/EBPβ knockout was confirmed by immunoblotting. Data in g represent mean ± s.d. of three mice per group; *P < 0.05, Student’s t-test. i Immunoblotting analysis of delta-secretase and C/EBPβ in age-matched wild-type, 5XFAD and 3xTg AD mice. Wild-type and AD mice of different ages were sacrificed and their brain lysates were subjected to western blotting analysis. j IL-6 is escalated in AD mouse models in an age-dependent manner. Wild-type and AD mice of different ages were sacrificed and their brain lysates were subjected to IL-6 ELISA analysis. Data represent mean ± s.e.m. of three independent experiments (*P < 0.05, one-way ANOVA)
