Fig. 3
From: Direct binding of CEP85 to STIL ensures robust PLK4 activation and efficient centriole assembly
CEP85 co-localizes with STIL at an early stage of centriole duplication. a, b IF analysis of CEP85 and STIL localization in U-2 OS cells after 24 h S-phase arrest using thymidine (1 mM). Selected images showing CEP85 and STIL labelling. b Bar graph, the percentage of centrioles with different localization patterns (n = 100/experiment, three independent experiments). Scale bar 0.5 μm. c 3D-SIM micrographs of S-phase arrested (1 mM thymidine) U-2 OS cells stained with the indicated antibodies. d, e 3D-SIM micrographs in U-2 OS Tet-inducible Myc-PLK4 cells after 24 h PLK4 induction and S-phase arrest using thymidine (1 mM), showing CEP85, STIL and Myc-PLK4 staining. PLK4 ring structures were encircled by white dashes. Scale bar 0.5 μm. e The graph indicates the fraction co-localization between CEP85 and STIL (n = 20/experiment, two independent experiments). All error bars represent SD
