Fig. 2
SCRaMbLE of Y12-synX rapidly improves thermotolerance. a Serial dilution assay comparing the growth of two independent SCRaMbLEd isolates of Y12-synX (yYW166 and yYW167) with the non-SCRaMbLEd parent strain (yMS423) and a Y12 diploid strain (yYW207) under various temperature conditions, as well as in YPGE. b Average sequencing depth per segment along synX of yMS423, yYW166, and yYW167. Deletions (boxes A, B, C, E, F, G, H, I, and J), as well as a duplication (box D) are highlighted. c BY4743, a Y12/Y12 diploid, yMS423, yYW166, yYW167, and yMS423∆ were all grown in liquid YPD overnight and diluted to a starting A600 of 0.1 in fresh YPD. These strains were then cultured in a 96-well plate reader with shaking at 30 °C or 42 °C. Optical density measurements were taken every 10 min and used to calculate doubling time. Error bars shown are mean and standard deviation from four technical replicates. One-way ANOVA with multiple comparisons was used to assess difference between each sample and yMS423 (***p < 0.001, **p < 0.01). Variance between the groups was determined to be similar
