Table 1 Strains used in this study

From: Heterozygous diploid and interspecies SCRaMbLEing

Strain name

Description

Genotype

BY4741

 

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0

BY4743

 

MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0

yYW117

BY4741 containing synX

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SYN10 ho::tR(ccu)J

yYW168

synX lys2::NatMX

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SYN10 ho::tR(ccu)J lys2::NatMX

yYW139

BY4741 containing synVsynX

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SYN5 SYN10 ho::tR(ccu)J

yYW169

synVX lys2::NatMX

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 SYN5 SYN10 ho::tR(ccu)J lys2::NatMX

yMS253

S288C alpha haploid

MATα ura3::KanMX ho::Hyg

yMS275

Y12 alpha haploid

MATα ura3::KanMX ho::Hyg

yMS354

CBS5829 alpha haploid

MATα ura3::KanMX ho::Hyg

yYW207

Y12 diploid

MATa/α ura3::KanMX/ura3::KanMX ho::Hyg/ho::Hyg

yYW208

CBS5829 diploid

MATa/α ura3::KanMX/ura3::KanMX ho::Hyg/ho::Hyg

yMS401

Diploid of yYW168 and yMS253

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yMS426

Diploid of yYW169 and yMS253

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN5/WT5 SYN10/WT10

yMS423

Diploid of yYW168 and yMS275

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yMS423∆

Diploid of yYW168 and yMS275 with deletion of YJL154C to YJL140W in synX

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yMS448

Diploid of yYW169 and yMS275

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN5/WT5 SYN10/WT10

yMS521

Diploid of yYW168 and yMS354

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yMS548

Diploid of yYW169 and yMS354

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN5/WT5 SYN10/WT10

yYW166

yMS423, SCRaMbLEd, heat tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yYW167

yMS423, SCRaMbLEd, heat tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yYW184

yMS521, SCRaMbLEd, caffeine tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yYW185

yMS521, SCRaMbLEd, caffeine tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yYW186

yMS548, SCRaMbLEd, caffeine tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN5/WT5 SYN10/WT10

yYW187

yMS548, SCRaMbLEd, caffeine tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN5/WT5 SYN10/WT10

yYW195

yMS548, SCRaMbLEd, caffeine tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN5/WT5 SYN10/WT10

yYW196

yMS548, SCRaMbLEd, caffeine tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN5/WT5 SYN10/WT10

yMS637

yMS521, SCRaMbLEd, caffeine tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yMS638

yMS521, SCRaMbLEd, caffeine tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yMS639

yMS521, SCRaMbLEd, caffeine tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yMS640

yMS521, SCRaMbLEd, caffeine tolerant

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yMS674

yMS521 + episomal POL32

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

yMS675

yMS521 + episomal POL32

MATa/α ura3Δ0/ura3::KanMX ho::tR(ccu)J/ho::Hyg lys2::NatMX/LYS2 SYN10/WT10

Back to article page