Fig. 1

Differentiation and division proliferation history of HSCs after transplantation into non-conditioned recipients. a HSCs (LSK CD48⁻CD41⁻CD150⁺) were labeled with CellTrace Violet dye and 3600 cells were transplanted into non-conditioned wild-type mice. Purity of transplanted cells was >99% for each experiment. b Bone marrow was harvested at 36 h after transplantation and donor cells were analyzed using the indicated gates. c Dilution of CellTrace Violet in donor LSK and MPs, 36 h after transplantation. Labeled and transplanted naive CD62L⁺CD4⁺ T cells were used as reference for undivided cells. Five hundred donor cells were analyzed from 11 transplanted mice, representative data for 1 out of 13 experiments. d Phenotype of undivided and divided donor MPs (n = 11), representative example of 13 independent experiments. e Frequency of restricted progenitors in undivided (0 div.) and divided (1 div.) donor MPs, pooled data from 13 independent experiments. Unpaired Student's t-test, data are means +/− s.d., ***P = 0.0002, *P = 0.02