Fig. 4
Cell cycle distribution of undivided donor HSCs, CMPs, PreMEs, and PreMegs. a Prediction of cell cycle phases for all undivided donor cells 36 h after transplantation. Shown is the average expression of G1 genes (x axis) and S/G2/M genes (y axes). b Violin density plots for the most differently expressed cell cycle genes. y Axis represents gene expression. The horizontal width of the plot shows the density of the data along the y axis. Statistical significance was determined using the Hurdle model. *P < 0.05, **P < 0.01, ***P < 0.0001. Exact P-value in supplemental Tables 2-3. c t-SNE plots for PreME/PreMeg cells based on cell cycle genes and MEP/platelet genes. d Mean expression of MEP/platelet genes was calculated for HSCs, PreMEs, and PreMegs in G0/G1 and S/G2/M phases and is depicted as fold increase relative to mean expression in HSCs in the G0/G1 phases. e RFP expression in undivided donor MPs at 36 h after transplantation of RFP− HSCs from Ki67RFP knock-in mice. Recipient MPs were used as negative controls for RFP expression. (representative example, n = 5, from 2 independent experiments)
