Fig. 7
Medium throughput dose response and single point antimalarial compound screening. a Dose response charts of inhibition of P. vivax and P. falciparum LS development following treatment with control compound KDU691 (a PI4K inhibitor) and reference compound atovaquone. b Dose response curves for six MMV portfolio drugs against both P. vivax and P. falciparum LS schizonts. c Activity of 913 Calibr Bioactive compounds against P. vivax in radical cure mode; activity for each compound is indexed on the X axis by plate position with individual plate control wells grouped and indicated. Monensin, active against P. vivax hypnozoites and schizonts in both prophylactic and radical cure modes, is indicated within a gray circle. d Dose response plots of inhibition of P. vivax LS parasites following treatment with three different ionophores or primaquine; PHH nuclei counts, normalized to DMSO controls, are shown to demonstrate selectivity index. Graph bars represent means with s.d. of biological replicates (n = 2) for DSM421, P218, AN13762, KAF156, pyrimethamine, and monensin; biological replicates (n = 3) for MMV390048 and atovaquone; or biological replicates (n = 4) for primaquine
