Fig. 5
Next-generation sequencing result of violacein SCRaMbLE-in strains. a Genome rearrangements in SCRaMbLE-in strains. Each SCRaMbLEd synII chromosome (770 kb) is represented as a sequence of arrows. The color of each arrow represents the segment number separated by loxPsym sites in the parental synII chromosome and the direction indicates the orientation. The unfilled arrow indicates exogenous violacein synthesis pathway. The red-outlined arrow represents the segment where the centromere is located. Based on the SCRaMbLE events, hierarchical clustering method was performed to cluster the strains to illustrate the component differences on the position and length. Remarkably a chromosome fusion event was observed in LWy256 and the total length of SCRaMbLEd synII is 1544.17 kb. b Large fragment duplications in the SCRaMbLE-in strains. Segment copy number in each strain is indicated as deletion and no copy (gray), one copy with no change (white), duplication as two copies (orange), and multiple duplications with more than three copies (red). The asterisk represents a CEN2 centromere resides in segment 8
