Fig. 6
Orthogonal inducible SCRaMbLE systems. a Design of SSR expression device and functional test device. SSR or SSR-LBD fusion protein were assembled into a pRS415-based centromeric vector with LEU2 as selection marker. URA3 marker was used as recombination reporter and flanked by two recombination sites. The reporter device was based on pRS413 centromeric vector with HIS3 as selection marker. b Orthogonal function test of Cre and Dre. Yeast cells with reporter device rox-URA3-rox did not survive with the expression of Dre and cells with reporter device loxP-URA3-loxP could not survive with the expression of Cre on SC-His-Leu-Ura plate. c Orthogonal induction test of CrePBD and DreEBD. Only cells with reporter device loxP-URA3-loxP and CrePBD under the induction of RU486, cells with reporter device rox-URA3-rox and Dre-EBD under the induction of β-estradiol did not survive on SC-His-Leu-Ura plate. PBD progesterone-binding domain, EBD estrogen-binding domain, E+ β-estradiol induction, R+ RU486 induction, SSR site-specific recombinase, LBD ligand-binding domain
