Table 2 Palindromic arm mutants of loxP

From: Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods

Lox mutant

Mutation element

Sequence

loxP

WT

ATAACTTCGTATAGCATACATTATACGAAGTTAT

lox71

LE

taccgTTCGTATAGCATACATTATACGAAGTTAT

lox66

RE

ATAACTTCGTATAGCATACATTATACGAAcggta

loxJT15

LE

AattaTTCGTATAGCATACATTATACGAAGTTAT

loxJT15 right

RE

ATAACTTCGTATAGCATACATTATACGAAtaatT

loxJT510

LE

taAcgTTCGTATAGCATACATTATACGAAGTTAT

loxJT510 right

RE

ATAACTTCGTATAGCATACATTATACGAAcgTta

loxJTZ17 left

LE

ATAAaTTgcTATAGCATACATTATACGAAGTTAT

loxJTZ17

RE

ATAACTTCGTATAGCATACATTATAgcAAtTTAT

  1. Uppercase indicates wild-type bases; lower case indicates mutation bases. Underline indicates spacer region
  2. LE left element, RE right element, WT wild type
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