Fig. 3
From: NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence
NOTCH1 signalling represses SAHF formation partially by repressing HMGA proteins. a, b qRT-PCR (n = 6) (a) and immunoblotting (b) for the indicated mRNA and proteins in IMR90 ER:HRASG12V cells stably infected with control vector or N1ICD-FLAG ± 100 nM 4OHT for 6 days. c, d qRT-PCR (n = 4) (c) and immunoblotting (d) of IMR90 cells expressing doxycycline (DOX)-inducible N1ICD-FLAG (iN1ICD) and infected with a mVenus control vector or dnMAML1-mVenus ± 1000 ng/mL DOX for 3 days. e Quantification of SAHFs in IMR90 cells expressing ER:HRASG12V, iN1ICD and EGFP or an EGFP-HMGA1 fusion ± 100 nM 4OHT for 6 days and ±1000 ng/mL DOX for 3 days. f qRT-PCR for the indicated mRNA in IMR90 cells expressing rThy1-mRFP and co-cultured with RPE1 cells expressing mVenus or JAG1-mVenus, isolated using MACS (n = 3). Statistical significance calculated using one-way ANOVA with Tukey’s correction for multiple comparisons (a, c) or two-sample t-test (e, f). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001
