Fig. 5
From: NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence
Ectopic N1ICD and HMGA1 knockdown antagonise chromatin opening in RIS. a Unbiased clustering of replicates for the conditions indicated. b, c Volcano plots showing regions of altered accessibility in N+RIS cells (b) and RIS+shHMGA1 (c) cells relative to RIS cells. Regions that are also opened in RIS (red) and NIS (purple) relative to growing cells are indicated. d Number of novel accessible regions in RIS (identified in Fig. 4c) that are repressed by N1ICD (significantly reduced in N+RIS vs. RIS) and are HMGA1 dependent (significantly reduced in RIS+shHMGA1 vs. RIS). The Venn diagram shows the number of regions repressed by N1ICD that are overlapped by a region dependent on HMGA1. e Genomic GC percentage of the accessible regions indicated. f K-means clustered heatmap showing the enrichment of normalised reads around accessible regions altered in any of the conditions relative to growing cells. g Genomic GC percentage of the clusters indicated, identified in f. e, g Mean ± s.d is plotted. Statistical significance calculated using one-way ANOVA with Tukey’s correction for multiple comparisons; ***p ≤ 0.001
