Fig. 2
From: Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity
High throughput droplet-mediated scRNAseq reveals additional epithelial cell states. a Overview for droplet-enabled scRNAseq approach as described above; basal and luminal epithelial cells were sorted together and subjected to combined scRNAseq analysis using the droplet-based scRNAseq. b Data from individual four was analyzed using Seurat and the distinct clusters (0–10) are displayed in tSNE projection with selected marker gene for each cluster, and main epithelial cell types (Basal, L1, L2) are outlined. Feature plots of characteristic markers for the three main cell types are shown on the right showing expression levels as gradient of purple. c Heatmap showing the top ten marker genes for each cluster as determined by Seurat analysis with three selected genes per cluster highlighted on the right. See Supplementary Fig. 2 for individual clustering and marker gene analyses for Individuals 5–7
