Fig. 4
From: Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity
Characterization and spatial integration of basal cell states. a Immunofluorescence analysis of ZEB1 protein expression (red) in combination with basal marker KRT14 (green) and DNA stain using DAPI (blue) within tissue sections from primary human reduction mammoplasty samples showing ZEB1 expression in a subpopulation of basal (KRT14+) cells. Scale bar = 15 µm. b Heatmap showing expression of genes previously shown to be up- (red) or down-regulated (blue) in a population of PROCR+ mammary stem cells show correlation with ZEB1+ cells in scRNAseq. c Immunofluorescence analysis of TCF4 protein expression (red) in combination with basal marker SMA (green) and DNA stain using DAPI (blue) within tissue sections from primary human reduction mammoplasty samples revealed that TCF4 is expressed in a subpopulation of basal (SMA+) cells. Scale bar = 25 µm. d Violin plot for expression of KRT14 by cell state showing highest expression in the myoepithelial (Myo) cells. e KRT14 and KRT8 double immunostaining revealed highest expression of KRT14 in ductal basal cells, while lobular basal cells show more diverse KRT14 positivity. Scale bar = 75 µm. See Supplementary Fig. 4 for violin plots displaying selected myoepithelial gene expression and identification of KRT8/KRT14 double positive cells
