Fig. 7
FXIIIA and fibrin cross-linking is associated with LUSC progression. a Representative IHC images (left) of tumors from the LUSC tissue microarray with low or high cross-linked fibrin (n = 96 patients). Kaplan-Meier plot of recurrent-free survival following surgical resection. P-values were obtained with the log-rank test. b Kaplan–Meier plot of overall survival in LUSC patients split by median expression levels of F13a1. P-values were obtained with the log-rank test. c Relative expression levels of F13a1 in THP1 monocytes stably expressing either F13 ORF or shRNAs against F13a1. P-values were obtained with Student’s t-test. d Schematic (left) and quantification of distant micro-metastases (right) following infusions of THP1 monocytes. NSG mice were injected via tail vein with LN4K1-OgNLuc cells on Day 1, followed by daily infusions of THP1 cells in the respective groups for Days 1–4. Mice were necropsied on Day 7, lungs were dissociated and FACS analysis performed for EpCAM + cells to quantify micro-metastases per lobe. Data are averages ± s.e.m. P-values were obtained with Student’s t-test, n = 3 mice/group. n.s. = non-significant, * P < 0.05, *** P < 0.001
