Fig. 3
Phf2 diverts glucose fluxes to protect liver from oxidative stress. Mice, injected with either GFP or Phf2 overexpressing adenovirus, were studied 3 weeks later in the fed state. a Glucose and oleate oxidation rate determined by measuring the production of 14CO2 from 14C-glucose or 14C-oleate for 4 h (n = 8 mice per group). b Basal glutamate/malate and succinate-driven mitochondrial respiration, which provide respectively electrons to the complex I and II of the mitochondrial reaction chain (n = 6 mice per group). c Relative ROS and carbonylated protein levels (n = 12 mice per group). d Metabolomic KEGG pathway enrichment analysis (n = 15 mice per group). e Heat map of metabolic intermediates of the PPP, serine, glycine, and GSH biosynthetic pathways (n = 15 mice per group). f Western blot analysis of proteins involved in oxidative stress defenses (n = 12 mice per group). g Liver NADPH content (n = 10 mice per group). h Measurement of Gpx activity (n = 10 mice per group). i Expression of genes involved in GSH synthesis (n = 10 mice per group). All error bars represent mean ± SEM. Statistical analyses were made using unpaired t-test. *P < 0.01 GFP compared to Phf2
