Fig. 2 | Nature Communications

Fig. 2

From: The rice blast resistance gene Ptr encodes an atypical protein required for broad-spectrum disease resistance

Fig. 2

CRISPR/Cas9-mediated mutation of Ptr in a resistant cultivar. a The Ptr gene was targeted with two specific gRNAs to achieve knockouts. CRISPR/Cas9 editing resulted in InDels of differing length or a fragment deletion between the two protospacer (PS) sites are indicated by asterisk (*). b Disease symptoms on wild-type Katy and CRISPR-edited Ptr mutant leaves after spray-inoculation with AVR-Pita-containing strains IB-49 (ML1) or IC-17 (ZN57). c Disease rating of Katy and Ptr mutant plants at 6 days post-inoculation with 0–5 rating scale where 0–2 is resistant and 3 to 5 is susceptible. d Average lesion length on Katy and Ptr mutant leaves at 6 days post-inoculation. Error bars indicate standard deviation (s.d.) of average lesion area and disease rating. Data represent means ± s.d. (n = 12)

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