Fig. 6
Lowered PTP opening threshold and increased cell death in SNCA triplication iPS derived neurons. a Representative images of mitochondria labelled with TMRM before ferutinin was added (0 min), immediately before mPTP opening (12 min), and after mPTP opening (20 min). b Representative traces of PTP opening in control and triplications in response to ferutinin. c Proportion of cells (%) that fully opened PTP in response to 25 μM ferutinin. N = 3 experiments, control n = 125 cells, and triplication n = 82 cells. d Representative traces of PTP opening in SNCA triplication neurons ± CsA in response to high laser. e Quantification of the time until laser-induced PTP opening in control (N = 3 experiments; n = 77 areas), SNCA triplication neurons (N = 3 experiments; n = 82 areas), and SNCA triplication neurons + CsA (N = 3 experiments; n = 49 areas). f Representative images and g quantification of cell death in control, isogenic control, and triplication neurons challenged with control buffer or oligomers ± CsA. N = 3 experiments, n ≥ 763 cells. g Scatter points represent individual fields of view. Two-tailed Student’s t-test for c. One-way ANOVA with Bonferroni correction (e, g). Scatter points represent sampled fields of view. Data represented as mean ± SEM. Scale bar = 10 μm. *p < 0.05; **p < 0.01; ***p < 0.001
