Fig. 3 | Nature Communications

Fig. 3

From: Pharmacologic inhibition of protein phosphatase-2A achieves durable immune-mediated antitumor activity when combined with PD-1 blockade

Fig. 3

PP2A inhibition and PD-1 blockade increase CD8+ activation in lymphoid organs and CD8+ infiltration in tumors. Balb/c mice in the respective groups were harvested 3 days after initiation of treatment and the spleen, tumor draining lymph nodes (dLN) and tumors were analyzed by flow cytometry. a Representative FACS plots of CD44 and CD62L in CD8+ T cells in the spleen. b Quantification of CD62-CD44+ (of CD8+ T cells) in the spleen and c tumor draining lymph nodes (n = 4–5). d Representative FACS plots of CD8+ CD3+ T cells as percentage of CD45+ cells. e–g Immune infiltrate analysis of e CD3+, f CD8+, and g CD4+ expressed as percentage of CD45+ cells (n = 5). h Ratio of CD8+ to CD4+ cells in tumor. i CD8+ CD44+ and j CD8+ Ki67+ expressed as percentage of CD45+ cells in tumor. *P < 0.05, (one-way ANOVA with Tukey’s multiple comparison test). Error bars depict SEM. Data represent one of two experiments with five independently analyzed mice/group

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