Fig. 6

LB-100 inhibits PP2A enzymatic activity and activates the mTORC1 pathway. CD3 T cells were isolated from mice splenocytes and cultured with or without stimulation using immobilized anti-CD3 (10 μg ml⁻¹) and soluble anti-CD28 (2 μg ml⁻¹). a PP2A enzymatic activity was measured after 3 h of activation. PP2A activity was measured as relative to activated control in presence of LB-100 dose titration. b Flow cytometry analyzing AKT phosphorylated at Thr308 (p-AKT(T308)) or Ser473 (p-AKT(S473)) after 3 h of stimulation in presence of LB-100 dose titration. c Flow cytometry analyzing phosphorylated S6 (p-S6) in presence of LB-100 dose titration. *P < 0.05, ***P < 0.001 (one-way ANOVA with Tukey’s multiple comparison test). Data are from one experiment representative of two independent experiments with similar results. Error bars depict SEM