Fig. 3
From: The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones
The RPAP3 C-terminal domain interacts with R2TP clients via RUVBL1/2 multimers. a Yeast two-hybrid analysis of interactions between RUVBL2 and RPAP3-Cter mutants. Alix is a negative control. ***: strong interaction; **: medium; *: weak; −: no interaction. b Molecular surface representation of RPAP3-Cter structure by specifying the location of the mutants that lost interaction with RUVBL1/2. c LUMIER assay showing the in vivo interaction between RPAP3-Cter and RUVBL1/2 mutant proteins. Top panel: schematic representation of the assay. Bottom panel: graph plotting the IP efficiency of the indicated proteins. The values are the IP efficiencies of the co-precipitation of the RL fusion proteins (IP/Input), normalized by the IP/Input values obtained with the anti-FLAG IP of the 3xFLAG-FFL fusion protein. Error bars: standard deviation. Stars: values significantly greater than six-times the mean value obtained in the control IPs without anti-FLAG antibody (Ct). **p-value < 0.001 (Z-test). d,e SILAC proteomic analysis of the partners of RPAP3-Cter-Mut1 and RPAP3-Cter-Mut2, respectively. Legend as in Fig. 2a
