Fig. 6
From: The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones
Identification of PIH1D2 partners. a Results of yeast two-hybrid screens using human PIH1D2 as bait and performed with human libraries from lung carcinoma cell lines and testis. The color of the lines indicate the strength of the Y2H interaction (PBS score). a red; b dark blue; c green; d light blue. Lines with two dots indicate that the prey was found in the two libraries. b Validation of the hits found in the yeast two-hybrid screens by LUMIER co-IP assays. The graph depicts the results of LUMIER co-IP assays performed with the indicated proteins. Error bars: standard deviation. Stars: values significantly greater than six-times the mean value obtained in the control IPs without anti-FLAG antibody (Ct). *p-value < 0.05; **p-value < 0.001 (Z-test)
